Optogenetic manipulation of neural circuits in awake marmosets.

TitleOptogenetic manipulation of neural circuits in awake marmosets.
Publication TypeJournal Article
Year of Publication2016
AuthorsMacDougall M, Nummela SU, Coop S, Disney A, Mitchell JF, Miller CT
JournalJ Neurophysiol
Date Published2016 09 01
KeywordsAction Potentials, Animals, Bacterial Proteins, Brain, Callithrix, Dependovirus, Female, Genetic Vectors, Luminescent Proteins, Microelectrodes, Models, Animal, Neural Pathways, Neurons, Optogenetics, Photic Stimulation, Rhodopsin, Serogroup, Wakefulness

Optogenetics has revolutionized the study of functional neuronal circuitry (Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. Nat Neurosci 8: 1263-1268, 2005; Deisseroth K. Nat Methods 8: 26-29, 2011). Although these techniques have been most successfully implemented in rodent models, they have the potential to be similarly impactful in studies of nonhuman primate brains. Common marmosets (Callithrix jacchus) have recently emerged as a candidate primate model for gene editing, providing a potentially powerful model for studies of neural circuitry and disease in primates. The application of viral transduction methods in marmosets for identifying and manipulating neuronal circuitry is a crucial step in developing this species for neuroscience research. In the present study we developed a novel, chronic method to successfully induce rapid photostimulation in individual cortical neurons transduced by adeno-associated virus to express channelrhodopsin (ChR2) in awake marmosets. We found that large proportions of neurons could be effectively photoactivated following viral transduction and that this procedure could be repeated for several months. These data suggest that techniques for viral transduction and optical manipulation of neuronal populations are suitable for marmosets and can be combined with existing behavioral preparations in the species to elucidate the functional neural circuitry underlying perceptual and cognitive processes.

Alternate JournalJ. Neurophysiol.
PubMed ID27334951
PubMed Central IDPMC5023415
Grant ListR21 MH104756 / MH / NIMH NIH HHS / United States
R01 DC012087 / DC / NIDCD NIH HHS / United States
IRG Funded