|Title||Imaging brain activity during complex social behaviors in Drosophila with Flyception2.|
|Publication Type||Journal Article|
|Year of Publication||2020|
|Authors||Grover D, Katsuki T, Li J, Dawkins TJ, Greenspan RJ|
|Date Published||2020 01 30|
|Keywords||Animals, Brain, Copulation, Courtship, Drosophila, Drosophila Proteins, Female, GABAergic Neurons, Male, Neuroimaging, Neurons, Olfactory Cortex, Sexual Behavior, Animal, Social Behavior|
Optical in vivo recordings from freely walking Drosophila are currently possible only for limited behaviors. Here, we expand the range of accessible behaviors with a retroreflective marker-based tracking and ratiometric brain imaging system, permitting brain activity imaging even in copulating male flies. We discover that P1 neurons, active during courtship, are inactive during copulation, whereas GABAergic mAL neurons remain active during copulation, suggesting a countervailing role of mAL in opposing P1 activity during mating.
|Alternate Journal||Nat Commun|
|PubMed Central ID||PMC6992788|
Imaging brain activity during complex social behaviors in Drosophila with Flyception2.